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CyFlow Space

  • Configurations with up to 13 fluorescence parameters and 3 physical light scattering parameters
  • Choice of lasers with 365, 375, 405, 488, 532, 561, 594, 638, 640 nm
  • Maximum acquisition rate up to 25,000 signals/s
  • Scatter particle size: 0.1 – 100 µm
  • Fluorescence sensitivity: < 100 MESF (FITC), < 50 MESF (PE)

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CyFlow Space – its flexibility gives you the space you need for your work

CyFlow Space – its flexibility gives you the space you need for your work

The CyFlow Space is a high-performance multi-laser flow cytometer, which offers the most flexible, simple and reliable features for routine and research work.

It is a compact multi-colour instrument for the analysis of individual cells and microscopic particles in suspension. The advanced flow cytometry technology of the CyFlow Space covers a wide range of different applications.

 

  • Fluorescence excitation by one to five light sources covering a range from ultra-violet to red excitation lines. o Analysis of up to 13 fluorescence parameters and three physical light scattering parameters from near UV to infra-red light. 
  • Analysis of cell and particle concentration by True Volumetric Absolute Counting (TVAC). 
  • Cell sorting by an optional cell and particle sorter. 
  • Fixed standard configurations and flexible customised configurations tailored to the user´s requirement. 
  • Walk-away automation by sample auto-loading from 96- and 384-well plates.
Software

Software

FloMax flow cytometry data acquisition and analysis software

The Windows™-based FloMax software includes multiple online gating, logical gates, and colour gating. N-colour online and offline software compensation is another standard feature of the FloMax software. Data may be acquired with compensation or as uncompensated raw data. Either way, compensation can be done, undone, or changed after analysis on stored data. User-selectable statistics are calculated on subset populations identified by regions that are created by the user with easy-to-use drawing tools, and combined using simple, logical formulas. Other sophisticated features include cell cycle analysis and peak analysis. Custom reports are quickly and easily generated from the acquired data using the integrated, macro-driven interface with Microsoft™ Word and Excel. FloMax data files are stored in FCS standard format and are therefore fully compatible with other commercial flow cytometry software.

The FloMax software is pre-installed on the Windows™ PC that is delivered as part of the CyFlow Space systems. Three additional licences for offline copies of the FloMax software are also included free of charge, which offers a cost-effective solution for working groups with the need for offline data analysis software.

Specification

Specification

Light sources:

Up to five light sources

  • Blue solid state laser: 50mW fix or 200mW adjustable at 488nm
  • Red diode laser: 25mW at 638nm or 40mW at 640nm
  • Violet diode laser: 100mW at 405nm
  • Green DPSS laser: 30 or 100mW at 532nm
  • Yellow laser: 100mW at 561nm
  • High-power UV LED: 365nm
  • Ultraviolet diode laser: 60mW at 375nm
  • Orange diode laser: 50mW at 594nm

 

Optics:

  • Modular optical system with up to 16/eight optical parameters with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL13
  • Exchangeable optical filters
  • Colour CCD camera for sample flow monitoring

 

Flow system:

  • Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
  • True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
  • Computer-controlled precision syringe pump, speed continuously adjustable from 0-20 µL/s

 

Software:

  • Microsoft Windows™ operating system with full network support, data analysis and real-time data display
  • Storage of instrument settings, screen layout templates, panels, gates and compensation matrices
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
  • Automated reporting of statistics, graphics and instrument settings to Word or Excel

 

Dimensions:

  • W 560mm x H 300mm x D 650mm
Upgrades

Upgrades

CyFlow Space Autoloading Station

General facts:

  • Automatic sample loading from 96-/384-well plates
  • High-speed mode < 15 minutes/96-well plate
  • User-defined cleaning and sample re-suspension
  • Start-up time < 2 minutes

 

Microplates:

  • 96- and 384-well plates with V-, U- and flat-bottom wells
  • Plates meeting the standards ANSI/SLAS (1-2004 through 4-2004) 

 

Sample volume:

  • Flexible choice of sample volume from 10 to 150µL

 

Speed:

  • Sample injection speed adjustable from 0.2µL/s to 10µL/s
  • Cycle time per microplate
    a. High-speed mode < 15min
    b. Balanced mode < 30min
    c. Precision mode < 50 min
    or customised by the operator

 

Software:

  • CyPADWindows™-based software

 

Dimensions:

  • W 200mm x H 280mm x D 320mm
Applications

Applications

Absolute cell and particle counting
Cell and particle sorting

Microbiology:

  • Yeast viability and fermentation process control
  • Water quality analysis
  • Absolute counting of bacteria and other microorganisms
  • Viability of bacteria and other microorganisms
  • Quality control in dairy industry and milk products
  • Marine biology and algae
  • Agrosciences and aquaculture
  • Quality control in cosmetics

 

Industrial applications:

  • Bioreactor process optimisation
  • Quality assurance in food and beverage industry
  • Biomonitoring
  • Biofuels

 

Cell Biology:

  • Cell cycle and DNA analysis
  • Viability and live/dead analysis
  • Cell proliferation
  • Apoptosis
  • Cancer research
  • Toxicology
  • Cytokines and bead-based assays
  • Cell signalling
  • Plant biology
  • Detection of ploidy level
  • Plant genome size and DNA analysis
  • Biomedical research 
  • Animal models
  • Stem cells
  • Immunology
  • Haematological disorders
  • Platelets
Complementary products

Complementary products

Sample preparation

Kontakt

Sysmex Austria GmbH

Tel.: +43 (1) 486 16 31-0
Fax: +43 (1) 486 16 31-25
office@sysmex.at

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