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CyFlow Ploidy Analyser

High-performance flow cytometry

The CyFlow Ploidy Analyser is a compact flow cytometer for ploidy analysis, high-resolution DNA and genome size analysis for plants, animals and micro-organisms.

This modular system is equipped with a UV LED (365 nm) and/or a green laser (532 nm) which, when used in combination with Partec CyStain reagents, provides high-resolution DNA analysis with DAPI or propidium iodide.

The CyFlow Ploidy instrument measures the DNA content of many thousands of individual nuclei within seconds or minutes. The result is directly displayed as a DNA histogram on the internal 15.4" colour display. This method is time- and cost-saving as it provides precise results quickly with an efficient and validated workflow. For higher throughput, it is possible to equip the system with an Autoloading Station that accepts up to two 96-well plates or 120 test tubes as a single load.

Features

Software

CyView: flow cytometry data acquisition and analysis software

  • Windows™-based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data

Upgrades

CyFlow Robby 6 Autoloading Station

General facts:

  • Protective housing with no light incidence 
  • Flexible well/tube measurements

Microplates and tubes:

  • 48- or 96-well plates, V-, U- and flat-bottom are fitting (max. two) 
  • Plates meeting the standards ANSI/SLAS (1-2004 through 4-2004) 
  • 2mL-tubes (max. 120)

Autoloader dimensions:

  • W 360mm x H 270mm x D 370mm

Applications

Absolute cell and particle counting

Agroscience and aquaculture:

  • Plant biology and algae

 Industrial applications:

  • Detection of ploidy level
  • Plant genome size and DNA analysis
Technische Spezifikationen
Light sources Up to two light sources
UV LED (365 nm)
Nd-YAG green laser at 532 nm (30 mW)
Optics Standard set-up and filters for propidium iodide (long pass filter 590 nm) and/or DAPI/SSC (long pass filter 435 nm)
Flow system Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
Completely closed fluidic system
Sample port with biosafety cleaning system
Software Windows™-based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display
Easy set-up of experimental templates (configuration files)
Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
Peak analysis function
Dimensions Stand-alone instrument: W 385mm x H 290mm x D 280mm
With Autoloading Station: W 745mm
With screen open: H 528mm
Kontakt

Sysmex Austria GmbH

Lienfeldergasse 31-33

1160 Wien

+43 1 486 16 31

+ 43 1 486 16 31 25

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Copyright © Sysmex Europe GmbH. All rights reserved.
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